Chromatin Proteomics (Imhof)

Department / Institute
Biomedical Center, Zentrallabor für Proteinanalytik
Subject area
Chromatin Proteomics
Project title
Systematic analysis of human methyltransferases and demethylases
Name of supervisor
Prof. Dr. Axel Imhof
Number of open positions
Language requirements
Proficiency in English
Academic requirements
Masters degree
Project time plan
Full Doctoral Study-Model: 36 or 48 months

Project description

Methylation of DNA, RNA and proteins is a major regulator of the flow of epigenetic information and enzymes mediating these modifications are frequently mutated in cancer. Protein methylating enzymes (protein methyltransferases, PMTs) as well as the ones removing this modification (protein demethylases, PDMs) use major intermediary metabolites as substrates thereby connecting the metabolic state of the cell with its information management. So far, we mainly focused our studies on histone methylation but evidence is mounting that non-histone substrates of PMTs and PDMs play equally important roles in this process. In this project we plan to systematically investigate the substrate specificity by investigating the protein methylome in a new panel of HEK293 cell lines we generated where individual methyltransferases and demethylases have been deleted using CRISPR/CAS technology. Within this project we plan to at a full molecular characterization of all 37 lines established so far with regards to their transcriptome, their proteome and their protein methylation patterns. The student will furthermore establish the panel as a powerful new tool to screen small molecule inhibitors directed against PMTs and PDMs.

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