Systematic analysis of proteinous autophagy cargo (Behrends)

Department / Institute
Munich Cluster for Systems Neurology (SyNergy)
Subject area
Proteomics, Cell Biology, Biochemistry
Project title
Systematic analysis of proteinous autophagy cargo
Name of supervisor
Professor Dr. Christian Behrends
Number of open positions
1
Language requirements
Proficiency in English
Academic requirements
Masters degree in Biological Sciences (i.e. Molecular Biology, Biochemistry, Cell Biology or similar). Applications must have excellent technical skills, an enthusiasm for using and developing new techniques, and the interpersonal ability to work with
Project time plan
Full Doctoral Study-Model: 36 or 48 months
Contact
Christian.Behrends@mail03.med.uni-muenchen.de

Project description

Autophagy is an umbrella term to describe a collection of mechanistically distinct lysosomal delivery pathways - macroautophagy, microautophagy and chaperone-mediated autophagy - that converge on surplus, defective or otherwise harmful intracellular proteins as common cargo. While autophagic delivery to lysosomal degradation is a major quality control guardian that helps to maintain homeostasis and dysfunctional autophagy contributes to the pathophysiology of human diseases such as neurodegeneration, comprehensive knowledge on proteinous autophagy cargo remains sparse. By exploiting leading expertise in systematic analyses of the autophagy system (Stadel et al. Molecular Cell 2015; Jia et al. Molecular Cell 2020; Zellner et al. Molecular Cell 2021) this project takes a cargo centric perspective and is focused on gaining a fundamental understanding of the contribution of autophagy pathways to proteostasis. Briefly, the student will employ advanced quantitative proximity and organellar proteomics to globally identify proteins that are delivered to lysosomes by the different autophagy pathways. Using extensive gene editing and pH-sensitive readouts, the student will subsequently dissect common and specific principles of autophagic engagement and delivery for a large spectrum of substrates.

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